The Concise Guide to Dermal Needling PDF 3rd Edition

There is a lot of science behind the results. Expectations and the results. Treatment rules and guidelines. Methods of treatment that complement one another. Warnings and things to think about. Avoiding the body’s inflammatory response People should avoid the ingredients in the products they want to buy. When it comes to “all things needling,” the Concise Guide to Dermal Needling pdf by Dr. Lance Setterfield is the best source. 

Many people think that they need a needling device and a cream to get results. This is why micro-needling has so many bad things happen, including the idea that “it doesn’t work.” The Concise Guide to Dermal Needling PDF, now in an Expanded Medical Edition, talks about the science of micro-needling and how to get results. The new edition of The Concise Guide to Dermal Needling PDF has 130+ more pages covering more topics, like PRP, LMW-HA, Stem Cells, and Paraben.

From the Author

Micro-needling has exploded in popularity and is now more popular than ever. Skincare experts want to learn more but are overwhelmed by contradicting information, which comes from sources with vested interests. Many people (both distributors and practitioners) overestimated the complexity of this therapeutic approach and were baffled when things went awry.

What is the advantage of micro-needling? How does one take advantage of the beneficial routes of cell-to-cell communication? Which micro-needling products are safe to use, and why? What conditions are we able to treat, and which ones respond best? What about the complexities? What features should we look for in a needling device? How much training will we require? Will we see a positive return on my investment? So, how about the FDA? How do we integrate this treatment into the services we already offer? Is there an additional risk as a result of this?

I’ve spent years exploring these concerns and training practitioners and clinics worldwide. In this new edition of The Concise Guide to Dermal Needling, I share my research and clinical expertise in the hopes of providing the answers you need. You will master the fundamentals of dermal needling and the advanced features of the modality, acquiring knowledge and confidence to work things out for yourself based on non-industry aligned information and a far more profound grasp of the science underlying this modality.

In this edition, I’ve spent a lot of time focusing on choosing the right patient and understanding their individual needs to maximize results and avoid issues. Most essential, in our quest to learn about micro-needling, we must keep in mind that there is a distinct individual (patient) on the other end of the device. This is referred to as the “5 P’s” of micro-needling success.

Finally, some people combine micro-needling with Stem Cells, PRP, Low Molecular Weight Hyaluronic Acid, and Parabens. The most recent information on these topics has been condensed into simpler terms to help remove the fog and give you a better understanding of these complex concerns.

Hopefully, by the end of this new pdf of The Concise Guide to Dermal Needling, your passion for skincare will be reaffirmed, and you will have earned the knowledge and confidence you need to provide the best treatments possible to your patients. You’ll gain respect as an expert, your business will thrive, and you’ll enjoy the adventure of meaningfully assisting your patients.

Contents of The Concise Guide to Dermal Needling PDF


What Is the Goal?
What Are the Features of Healthy Skin?
What Are the Benefits of Healthy Skin?
What Are the Clinical Benefits of Healthy Skin?
What Can We Do to Optimize Skin Health?
What Are the Key Goals of Skin Rejuvenation?
Regulating Cell Function
Regulating Keratinocyte Function
Regulating Melanocyte Function
Regulating Fibroblast Function
Features of Ablative and Non-Ablative Treatments

CHAPTER 2: Cell Function

Regulating Cell Function to Optimize Skin Health
What Nutrients Are Essential for Regulating Cell Function?
Keratinocyte Requirements for Optimal Function
Product Ingredients Affecting Keratinocytes
Antioxidants to Prevent Lipid Peroxidation
Melanocyte Requirements for Melanogenesis
Product Ingredients Affecting Melanogenesis
Fibroblast Requirements for Collagenesis
Vitamin C and Collagen Formation
Vitamin C and Other Functions
Vitamin A and Collagen Formation
Key Amino Acids for Collagen Synthesis: Proline and Lysine
Copper Peptides for Collagen Synthesis
Other Peptides that Target Collagen Synthesis
Zinc, a Co-Factor in Collagen Synthesis
Zinc Fingers
Bioflavonoids for Collagen Synthesis
Iron as a Co-Factor
Silicon, Magnesium, Selenium, and Calcium
Alpha-Linolenic and Linoleic Acid (Omega 3 and 6)
Stem Cells
Product Ingredients affecting Fibroblasts
What Growth Factors Regulate Cell Function?
Stem Cells

  • Navigating the New Frontier
    The History of Stem Cells
    What Are Stem Cells?
    Types of Stem Cells
    Embryo Stem Cells
    Fetal Stem Cells
    Adult Stem Cells (Autologous Sources)
    Making a Stem Cell Product
    Going Back to the Beginning
  • Dedifferentiation
    Do Plant Stem Cells Fit into the Picture?
    When is a Plant Stem Cell not a Plant Stem Cell?
    Back to Humans Stem Cells
    Bone Marrow Stem Cells
    Adipose Stem Cells
    What About Products Claiming to “Stimulate” Our Own Stem Cells?
    Are “Growth Factor” Products Also from Stem Cells?
    Bringing It All Together
    Comparisons – Embryo vs. AdultDistinctive Cellular and Molecular Features of Embryo Wound Healing
    Differences Between Wound Healing in Embryos and Adults
    Other Differences and Potential Influences Unique to Embryonic Wound Healing
    The Adult Wound Healing Process
    The Phases of Wound Healing
    Coagulation Phase (If Ablative)
    Inflammatory Phase (1-3 Days)
    Lag & Proliferative Phase (3-5 Days)
    Fibroplastic Phase (5-20 Days)
    Maturative and Remodell ing Phase (28 Days-2 Years)
    Plausible Explanations for the Differences Between the Embryo and Adult Wound Healing Models
    Growth Factors
    The Role of Hyaluronic Acid
    Why Does Fibrosis Result with IPL, Laser, Fraxel®, Thermage®, Etc?
    Conclusions and Significance
    What It Is and What It Can Do
    Needling Is About Far More Than Collagen Induction
    Needling Devices/Methods for Collagen Induction
    Tattoo Devices
    RF Combined with Microneedling
    Electronic Devices
    How to Select a Motorized Needling Device
  1. PATIENT SAFETY – Cross Contamination
    Rollers vs. Electronic Devices
    Studies Done with Rollers Cannot Necessarily be Extrapolated to Pens
    Advantages of Electronic Devices
    Advantages of Rollers
    Myths Propagated by Marketing
    FDA Reclassification of “Pens” as a Medical Device
    Cosmetic vs. Medical Need ling
    Differences in Needle Size
    Cosmetic Needling: How Does It Work?
    Effects on the Epidermis
    Effects on the Dermis
    Dermal-Epidermal Junction – A New Frontier
    Medical Needling: How Does It Work?
    Pinpoint Accuracy – Taking Skin Rejuvenation to a New Level?
    General Observations Opposing Conventional Wisdom
    Structure of the Dermal-Epidermal Junction
    Which Cells Are Responsible for Production of Basal Membrane Components?
    Components of the DEJ and Associated Genes Showing Influence of Microneedling
    Anchoring Filaments
    Lamina Densa
    Why Cosmetic Needling May Improve Wrinkles
    New Indications/Applications for Microneedling
    Active Acne
    Atopic Dermatitis
    Melanocyte Stem Cell Transplants
    Melanin Deposits in Hair Follicles to Enhance Laser Hair Removal
    ALA for Treating Actinic Keratoses
    ALA for Photodynamic Photorejuvenation
    Microneedling vs. Microinjection of Tranexamic Acid for Treating Melasma
    Microneedling with Bleomycin for Treatment of Verruca
    What Are the Most Effective Uses of Dermal Needling?
    How Needling Improves Various Skin Disorders & Conditions
    Lax Skin and Wrinkles
    What Are Some of the Causes of Lax Skin and Wrinkles?
    How Does Needling Correct Lax Skin & Wrinkles?
    UV Damage
    What Are Some of the Causes for This Failed Repair or Destruction/Removal?
    How Does Needling Correct UV Damage?
    What Are Some of the Causes for Hyperpigmentation?
    How Does Needling Correct Hyperpigmentation?
    What Are Some of the Causes for This?
    How Does Needling Correct Scars?
    Stretch Marks
    Histologica l Findings
    What Are Some of the Causes for Stretch Marks?
    How Does Needling Correct Stretch Marks?
    What Are Some of the Aggravating Factors of This Condition?
    How Does Needling Correct Rosacea?
    Hair Loss
    Androgenetic Alopecia
    Alopecia Areata
    What Are Some of the Causes for Hair Loss?
    How Does Needling Correct Hair Loss?
    Synergistic Products for Hair Restoration in Conjunction with Needling
    Patient History and Exam
    Consultation and Treatment Records
    Contra-Indications/Special Precautions
    Atopy, Asthma, Hay Fever and Allergies
    Anticoagulated Patients
    Drugs and Supplements Associated with Photosensitivity
    Considerations with Fitzpatrick III and Above
    Ingredients to Avoid with Dermal Needling
    Citrus-Derived Preservatives
    Formaldehyde-Releasing Preservatives
    Other Preservatives
    High Risk with Needling
    Alpha Hydroxy Acids or AHAs
    Beta Hydroxy Acids or BHAs
    Other Acids
    Dyes and Colouring Agents
    UV Filters
    The Collagenase Story (Another Precaution)
    Avoiding the Inflammatory Response
    Back to Basics – Normal Maintenance and Repair in Unwounded Skin
    What Makes a Fibroblast Tick?
    Back to Basics – Collagen
    Back to Basics – Elastin
    Back to Basics – Repair in Wounded Skin
    Which Wound Healing Phases Are Necessary in Non-Ablative Rejuvenation Treatments?
    Fibroblasts – Good or Bad?
    Myofibroblasts -The Bad Guys
    What Are the Four Possibilities of Response to Injury?
    A Brief Review of Collagen During Acute Wound Healing
    A Brief Review of Elastin During Acute Wound Healing
    Why the Fuss? Things Look Great!
    Back to Basics – Keratinocyte-Fibroblast Interaction
    Combating Infiammation as Part of the Inevitable Rejuvenation Process
    Platelet Rich Plasma
    Safety with Neuromodulators and Fillers
    IPL, Laser, Fraxel®, Etc.
    Risk of Secondary Infection with Cosmetic Needling
    Immune-Suppressed Patients
    Skin Preparation – Home (Cosmetic Needling)
    Home Care Products
    Cosmetic Needling (Rolling) at Home
    Cosmetic Roller Instructions
    Step 1: Cleansing
    Step 2: Roller
    Step 3: Active Ingredients Application
    Step 4: Protection
    Step 5: Roller Maintenance at Home
    Skin Preparation
  • Clinic (Medical Needling)
    Topical Anaesthesia vs. Dental Blocks
    What Size Needle?
    Clinical Techniques
    Medical Needling Protocol
    Best Practice Needling Technique and Procedures Using a Roller
    Best Practice Needling Technique and Procedures Using a Pen
  1. Technique for Even Endpoint (Erythema)
  2. Below the Eyes
  3. Perioral Lip Lines
  4. Nasolabial and Mental Folds
  5. Nose
  6. Forehead
  7. Upper Lids
  8. Wrinkles in Front of Ears
  9. Neck
    1 0. Scars
    End Point with Microneedling Treatments
    Number of Passes
    Visible Endpoint
    Adjuncts: Platelet Therapy
    How to Clean the Medical Needling Device
    Post Treatment Considerations
    Immediate Post Treatment Care
    Home Care Considerations
    Predicted Appearance After Medical Needling
    Post Treatment Plan Guideline
    New Post-Microneedling Protocols
    Immediately after Microneedling
  • Collagen Induction and Scars
    Inflammatory Phase 1 -5 Days
    Fibroplastic Phase 5-14 Days
    Maturative Phase 1 4-28 Days to 2 Years
    Immediately after Microneedling
  • Hyperpigmentation
    Bringing It All Together to Avoid Failure in Microneedling Treatments
  • Picking the Fights you can Win
    Is This the Appropriate Patient to Treat?
    Exam and Diagnosis (Depth of Pathology)
    Diagnostic Equipment
    Magnifying Lamp
    Wood’s Light
    Photographic Equipment
  1. Appropriate Product (Ingredient) Selection
  2. Appropriate Pen Selection
  3. Appropriate Penetration Depth – Needle Length
  4. Appropriate Period Between Treatments (Frequency)
    Required Number of Treatments
    Scope of Practice
    Am I qualified to treat this condition?
    Will this condition respond to needling?
    Para ben Story Update
    Signalling Molecules
    Cell Receptors
    “Imposters” – Endocrine Disruptors
    Signalling Molecules – Hormones
    Breast Cell Receptors
    Breast Cell Receptors – Hormonal
    Breast Cell Receptors – Non-Hormonal
    Receptors and Breast Cancer
    HER2 Overexpression in Breast Cancer
    Simplified Clinical Relevance of Receptors and Breast Cancer
    Why Have Many Studies Failed to Prove Paraben Links to Breast Cancer?
    Myth – Estrogenic Effect Associated with Parabens is Based on Estrogen Mimicry
    If Not Direct Stimulation of ERs by Parabens, Perhaps Via HER2 Pathway??? – No!
    Inching Closer to the Truth – Study Looks at “Parabens and Human Epidermal Growth
    Factor Receptor Ligand Cross-Talk in Breast Cancer Cells”
    Bringing It All Together
    Heregulin (HRG)
    Where Do We Go from Here?
    HA – No Laughing Matter
    Hyaluronic Acid – Beyond Wrinkles, Moisturising, and Plumping
    How is Hyaluronic Acid Made?
    How is Hyaluronic Acid Fragmented?
    Causes of HA Fragmentation
    Biofeedback to Facilitate Homeostasis – HA Fragmentation
    Biofeedback to Facilitate Wound Healing – HA Fragmentation
    What Are Some of the Functions of Hyaluronic Acid?
    Are the Antioxidant Properties of LMW-HA Superior to HMW-HA?
    Hyaluronic Acid Directing “Life and Death” via Hyaluronic Receptors
    Marketing Spin vs. Evidence-Based Science – Why the Disconnect?
    The Biological Function of HA Molecules Based on Size Differs Drastically
    Pathologies Associated with LMW-HA
    But We Are Only Applying HA to the Epidermis. Surely There Are No Systemic Effects?
    Does Dermal Infusion Occur When Products Are Applied Topically?
    Are There Biological Effects at an Epidermal Level That Induce Dermal
    Effects or Systemic Effects?
    What About Contradictory Studies?
    Surely Products Approved by Health Agencies Meet Safety Specifications?
    But the Results Are Great!
    If Injury Creates LMW-HA, Why is it Safe to Microneedle?
    The Platelet-Rich Plasma Story
    What is PRP?
    The Upside of PRP
    PRP and the FDA
    How Does PRP Work?
    How is PRP Prepared and Does It Really Matter?
    History of PRP
    PRP Uses
    PRP Use in Veterinary Medicine
    PRP Use in Orthopedics and Sport Medicine
    PRP Use in Dental Surgery
    PRP Use in Dermatology – General
    PRP Use in Dermatology – Alopecia
    General Discussion
  5. Impossible to standardize – Too many variables
  6. Disrupting the balance (Homeostasis) of nature – Agonists and Antagonists
  7. “Shotgun Therapy” vs. “Cruise Missiles”
  8. Flawed evidence – When is a “study” not a study?
    The Downside of PRP
    PRP Studies – Past and Future
    PRP – Fact or Fiction?
    PRP – What Does the Future Hold?
    Expectations and Outcomes
    UV Damage
    Lax Skin
    Actinic Keratosis
    Stretch Marks
    Hair Loss
    Adverse Reactions
    Review of Complications with Microneedling
    Cross Contamination
    Allergic Reactions
    Post-Inflammatory Hyperpigmentation
    Nerve Injuries
    Photocontact Dermatitis
    Case Study (In the Patient’s Own Words)
    The How’s, When’s & Why’s of Combination Therapy
    The Concept of Corneotherapy – Back to the Future
    Treatment Outline
    Non-Ablative Treatments
    Light-Emitting Diode (LED)
    Lymph Drainage
    Intense Pulsed Light (IPL)
    Mid-Infrared Lasers
    Ablative Treatments
    Chemical Peels
    Alpha Hydroxy Acids (AHA)
    Lactic Acid
    Mandelic Acid (Alpha-Hydroxybenzeneacetic Acid)
    Beta Hydroxy Acids (BHA)
    Salicylic Acid
    Beta-Lipohydroxy Acid (C8-Lipohydroxy Acid) (LHA)
    Other Acids
    Trichloroacetic Acid (TCA)
    Bromelain or Papaine
    Desquamation Due to Epidermal Cell Turnover vs. Exfoliation Due to Ablation
    Ablative Treatments (Continued)
    Microdermabrasion (MDA)
    Laser Resurfacing (Ablative)
    Pulsed Dye Laser
    Infrared Lasers
    Other Modal ities
    Hyperbaric Oxygen Therapy
    Radio Frequency (RF)
    Summary of Appropriate Combination Therapy
    Why Consider Microneedling in Your Practice?
    The Role of Keratinocytes in Collagen Induction
    Frequently Asked Questions
    Q&A: Interesting Question and Answer
    Author’s Response
    Patient’s Response

Author of The Concise Guide to Dermal Needling

Dr. Lance Setterfield graduated from the University of Stellenbosch in Cape Town, South Africa, in 1 983 and emigrated to Canada in 1 988 after completing five years of post-graduate training. His personal experience with skin cancer prompted a specific interest in the subjects of cancer, skin health, and anti-aging. Dermatology accounted for a significant portion of his practice in rural Canada.

One of the first doctors in Canada to use medical needling in his practice was Dr. Setterfield. He started his clinic in 2004 to study the effects of needling on the skin with pharmaceutical and homeopathic medications, plant extracts, vitamins, and other ingredients, after working as a co-founder and medical director of laser clinics in the Victoria area. His main focus has been on cell-to-cell communication, specifically the effects of dermal needling on the epidermis and dermis, emphasizing the significance of epidermal injury as an alternative to dermal injury rejuvenation.

With this paradigm shift toward the epidermis and away from the concept of prolonging inflammation to stimulate collagen creation, his study resulted in a partnership with clinics and firms all over the world, allowing him to be at the cutting edge of micro-needling technology. “Dermal Needling and Wound Healing” is a subject many people want to learn more about, so he made training courses and started a lecture tour to teach people about it. He did this in countries like the UK, the US, and Canada.

Dr. Setterfield wrote the first book on dermal needling (201 0), “The Concise Guide to Dermal Needling – Medical Edition,” which was later updated in 201 3 as an “Expanded” edition. This current book (2017) is a “Revised and Expanded” version with significant information on critical areas in the cosmetic industry about micro-needling. There is also a Spanish version available. Dermal needling has grown in popularity rapidly, and thousands of practitioners worldwide have benefited from the instruction he gives through his books, Online Microneedling Course, and live classes. (See for further information.)

Pros & Cons

  • The Concise Guide to Dermal Needling PDF is quite educational about the dos and don’ts of micro-needling. Because the book is geared toward professionals (doctors and estheticians), certain sections read like biochemistry or medical textbook, but there is also a wealth of specific practical knowledge. Dr. Setterfield is an expert, and he has kindly shared his experience and expertise with all of his readers.

  • This book contains a lot of information. However, the print is tiny. It’s so little that I can barely read it! Even if you have glasses, you will have difficulty reading this. Be cautious.

Book details

  • Book Name: The Concise Guide to Dermal Needling Third Medical Edition – Revised & Expanded
  • Publisher: ‎ Acacia Dermacare; 3rd edition (December 22, 2017)
  • Language: ‎ English
  • Paperback‏: ‎ 296 pages
  • ISBN-10‏: ‎ 0992060419
  • ISBN-13‏: ‎ 978-0992060411
  • Item Weight: ‎ 1.62 pounds

Download The PDF of The Concise Guide to Dermal Needling 3rd Edition

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